Description
Aviva Systems Biology SLC16A7 ELISA Kit (Human) (OKEH06594) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-SLC16A7 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated SLC16A7 and incubated. The SLC16A7 found in the sample or standards competes with the biotinylated SLC16A7 for limited binding sites on the immobilized anti-SLC16A7 antibody. Excess unbound biotinylated SLC16A7 and sample or standard SLC16A7 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated SLC16A7 captured in the well and inversely proportional to the amount of SLC16A7 which was contained in the sample or standard